PreSeed does not cause a significant decrease in progressive sperm motility or chromatin integrity in contrast to other lubricants used by couples.
Ashok Agarwal Ph.D.a, Fnu Deepinder M.D.a, Marcello Cocuzza M.D.a, Robert A. Short Ph.D.b and Donald P. Evenson Ph.D.c
Fertility and Sterility : Volume 89, Issue 2, February 2008, Pages 375-379
Objective
To evaluate the effect of vaginal lubricants PreSeed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity.
Design
Prospective, comparative, in vitro study.
Setting
Andrology laboratory at tertiary care hospital.
Patient(s)
Thirteen normozoospermic donors.
Intervention(s)
Semen samples from 13 subjects were incubated in human tubal fluid media (HTF) controls and 10% (vol/vol) of PreSeed, FemGlide, Astroglide, and Replens lubricants. After 30 minutes, progressive sperm motility was assessed by light microscopy. Semen samples of 12 patients were placed in positive control (HTF), negative control (10% K-Y Jelly lubricant), and 10% vol/vol PreSeed and FemGlide lubricants. After 4 hours culture, spermatozoa were analyzed for percent DNA fragmentation index with use of the acridine orange-based sperm chromatin structure assay.
Main Outcome Measure(s)
Sperm motility and percent DNA fragmentation index.
Results
Percent motility did not differ significantly between HTF controls and PreSeed, whereas FemGlide, Replens, and Astroglide lubricants demonstrated a significant decrease in motility. There was no significant difference in percent DNA fragmentation index between the HTF controls and PreSeed, but a significant decline in sperm chromatin quality occurred with FemGlide and K-Y Jelly.
Conclusion
PreSeed does not cause a significant decrease in progressive sperm motility or chromatin integrity in contrast to other lubricants used by couples.